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KMID : 0364219690120030085
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Korean Journal of Zoology
1969 Volume.12 No. 3 p.85 ~ p.93
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Studies on the Effects of Steroids on DNA Synthesis of Chromosmoes in Synchronized Human Cells
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Kang Yung-Sun
Park Sang-Dai
Ryu Chung-Hee
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Abstract
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The frequencies of chromosomal aberrations, unmerical variations at various time intervals and DNA synthetic patterns after the treatment with steroids in synchronized human kidney cells treated with 5-AU were investigated in the present experiment. 1. In 5-AU treated group, the frequency of chromosomal aberrations per cell was 0.131, 3 times of control group. In 5-AU + progesterone and 5-AU + testosterone groups, the frequency of chromosomal aberrations per cell was 0.340 and 0.452 respectively. 2. In 5-AU treated group, the frequency of cells with abnormal chromosome number was 0.8%, which was distributed throughout the time regardless of time interval. In 5-AU + progesterone and 5-AU + testosterone groups, the frequencies of cells with abnormal chromosome number were 2.2% and 4.3% respectively and they increased with the time. In 5-AU + progesterone group, the frequency of chromosomal aberrations exhiited the peaks at 12 and 18 hour stage after the treatment with steroids and, in 5-AU + testosterone group, it decreased with the time and in 5-AU treated group no significant difference was observed 3. The increase of labeled metaphases and labelling intensities in 5-AU treated cells are the result of the accumulation of cells at S stage by 5-AU. The decrease of labeled metaphases, labelling intensities and the delay of DNA synthetic time were observed in steroid groups. DNA synthetic pattern of sex chromosomes differs according to the step of cell cycle and DNA synthetic time is irregular because of double treatments with 5-AU and steroids.
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